Scientific Research on NMN and NAD+

Nicotinamide Mononucleotide (NMN) and Nicotinamide Adenine Dinucleotide (NAD+) are essential molecules involved in energy metabolism, cellular regeneration, and DNA repair. As we age, NAD+ levels in the body naturally decline, leading to reduced mitochondrial efficiency, lower energy levels, and accelerated cellular aging.

Scientific research around the world is focused on understanding the role of NMN and NAD+ in the human body and their potential impact on health, longevity, and metabolic function. On this page, we present an overview of publicly available scientific studies that shed light on the mechanisms of action of these compounds.

Evaluation of the Anti-Aging Effects and Safety of NMN in a Randomized, Controlled Clinical Trial

Methodology:

Participants: 66 healthy individuals aged 40 to 65.

Intervention: Daily intake of two capsules containing 150 mg of NMN each, or placebo (starch), for 60 days.

Study Design: Double-blind, parallel-group, placebo-controlled trial.

Results:


Significant increase in NAD+ levels in the NMN group compared to placebo.


Improvement in functions associated with aging.


NMN was well tolerated; no serious adverse events were reported.

Conclusion:

NMN supplementation may be an effective and safe approach to counteract aging-related effects in middle-aged and older adults.

Huang H (2022) A Multicentre, Randomized, Double Blind, Parallel Design, Placebo Controlled Study to Evaluate the Efficacy and Safety of Uthever (NMN Supplement), an Orally Administered Supplementation in Middle Aged and Older Adults.

Front. Aging 3:851698. doi: 10.3389/fragi.2022.851698

Evaluation of the Impact of NMN Supplementation on Aerobic Capacity in Young and Intermediate-Level Amateur Runners

Methodology:

Participants: 48 healthy, recreationally trained runners aged 27–50 years.

Intervention: Six-week NMN supplementation in three different dosages.

Training: Participants trained 5–6 times per week for 40–60 minutes.

Assessment: Cardiopulmonary exercise tests conducted at baseline and after 6 weeks.

Results:

Aerobic Capacity: Groups receiving medium and high doses of NMN showed a significant increase in oxygen uptake (VO₂) and power output at the first and second ventilatory thresholds compared to the placebo group.

Safety: NMN supplementation was well tolerated; no serious adverse effects were reported.

Conclusion:

NMN supplementation combined with regular training may enhance aerobic performance in amateur runners, likely by improving oxygen utilization in skeletal muscles.

Liao, B., Zhao, Y., Wang, D., Zhang, X., Hao, X., & Hu, M. (2021). Nicotinamide mononucleotide supplementation enhances aerobic capacity in amateur runners: a randomized, double-blind study.

Journal of the International Society of Sports Nutrition, 18(1).

https://doi.org/10.1186/s12970-021-00442-4

Evaluation of the impact of 12-week NMN supplementation on NAD+ metabolism and metabolic health parameters, including arterial stiffness, in healthy middle-aged adults.

Methodology:

Participants: Healthy middle-aged adults.

Intervention: 12-week oral NMN supplementation.

Assessment: NAD⁺ metabolism parameters and arterial stiffness measured by pulse wave velocity.

Results:

NAD⁺ Metabolism: NMN supplementation effectively increased NAD⁺ levels in participants.

Arterial Stiffness: Potential benefits were observed in the form of reduced arterial stiffness, suggesting improved vascular function.

Conclusions:

Long-term NMN supplementation is safe and effectively enhances NAD⁺ metabolism in healthy middle-aged adults. Additionally, NMN may offer vascular health benefits by reducing arterial stiffness.

Katayoshi, T., Uehata, S., Nakashima, N. et al. Nicotinamide adenine dinucleotide metabolism and arterial stiffness after long-term nicotinamide mononucleotide supplementation: a randomized, double-blind, placebo-controlled trial.

Sci Rep 13, 2786 (2023).

https://doi.org/10.1038/s41598-023-29787-3

Evaluation of the effects of NMN supplementation on skeletal muscle insulin sensitivity in postmenopausal women with prediabetes.

Methodology:

Participants: 25 postmenopausal women with overweight or obesity and prediabetes.

Intervention: 10-week randomized, double-blind, placebo-controlled clinical trial.

Assessment: Insulin sensitivity measured using a hyperinsulinemic-euglycemic clamp and analysis of insulin signaling in muscle tissue.

Results:


  • Significant improvement in the NMN group compared to placebo.

    Increased phosphorylation of protein kinase AKT and mTOR in the NMN group.

    Elevated expression of genes associated with muscle remodeling in the NMN group.

Conclusion:

NMN supplementation improves skeletal muscle insulin sensitivity in postmenopausal women with prediabetes, suggesting potential benefits in the prevention of type 2 diabetes.

Yoshino, M., Yoshino, J., Kayser, B. D., Patti, G. J., Franczyk, M. P., Mills, K. F., Sindelar, M., Pietka, T., Patterson, B. W., Imai, S. I., & Klein, S. (2021). Nicotinamide mononucleotide increases muscle insulin sensitivity in prediabetic women.

Science (New York, NY), 372(6547), 1224–1229.
https://doi.org/10.1126/science.abe9985